s473 (Cell Signaling Technology Inc)
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99
Structured Review
Cell Signaling Technology Inc
s473
S473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 32615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+akt/Phospho-Akt+(Ser473)+Antibody/pmc12816905-99-32-35
Average 99 stars, based on 32615 article reviews
S473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 32615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+akt/Phospho-Akt+(Ser473)+Antibody/pmc12816905-99-32-35
Average 99 stars, based on 32615 article reviews
s473 - by Bioz Stars,
2026-10
99/100 stars
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other:Article Title: 2 H -pyrazolo[3,4- d ]pyrimidin-4-amine derivatives as novel selective fibroblast growth factor receptor 2 (FGFR2) inhibitors. Article Snippet: Primary antibodies FGFR2(23328s), phospho‐FGFR (3471s), phospho‐FRs2α (3861s), eRK1/2 (4695s), phospho‐eRK1/2 (4370s), akt (pan) (11e7) (4685s), phospho‐aKt (9271s), GaPDh (5174), and Article Title: Phosphatidylserine-Rich circulating extracellular vesicles activate TAM receptor signaling to promote skin wound repair. Article Snippet: Phosphatidylserine (PS) is an anionic phospholipid that acquires signaling activity when exposed on membrane surfaces.. Although small extracellular vesicles (sEVs) have been implicated in diverse biological processes, the contribution of membrane-associated PS to vesicle-mediated signaling remains incompletely understood.. Here, we show that circulating small extracellular vesicles (csEVs) activate dermal fibroblasts and keratinocytes through a PS-dependent receptor signaling mechanism. Article Title: Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathway Article Snippet: The following primary antibodies were used for analysis: bcl2 (rabbit, 1:1000, Proteintech, Cat# 12789-1-AP, RRID: AB_2227948), bax (rabbit, 1:1000, Proteintech, Cat# 50599-2-Ig, RRID: AB_2061561), coiled-coil myosin-like bcl2-interacting protein (beclin1; rabbit, 1:1000, Proteintech, Cat# 11306-1-AP, RRID: AB_2259061), sequestosome-1/ubiquitin-binding protein p62 (SQSTM1/p62; rabbit, 1:1000, Abclonal, Cat# A11250, RRID: AB_2758477), microtubule-associated proteins 1A/1B light chain 3B (LC3; rabbit, 1:1000, Abcam, Cat# ab48394, RRID: AB_881433), endoplasmic reticulum chaperone BiP (Grp78/BIP; mouse, 1:1000, Proteintech, Cat# 66574-1-Ig, RRID: AB_2881934), cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6; rabbit, 1:1000, Proteintech, Cat# 24169-1-AP, RRID: AB_2876891), PRKR-like Article Title: 2 H -pyrazolo[3,4- d ]pyrimidin-4-amine derivatives as novel selective fibroblast growth factor receptor 2 (FGFR2) inhibitors Article Snippet: Primary antibodies FGFR2(23328S), phospho‐FGFR (3471S), phospho‐FRS2α (3861S), ERK1/2 (4695S), phospho‐ERK1/2 (4370S), Akt (pan) (11E7) (4685S), phospho‐AKT (9271S), GAPDH (5174), and Control:Article Title: Muscle Loss Driven by Extracellular Signal-Regulated Kinase Suppression via β-Adrenergic Activation in High-Normal Catecholamine Status Article Snippet: Following three additional washes with 1× TBST, protein bands were visualized using enhanced chemiluminescence solution and quantified using the ChemiDoc imaging system (Thermo Fisher, Waltham, MA, USA; iBright CL1500). .. Primary antibodies used included Incubation:Article Title: Induction of adipocyte thermogenic program by Rho-associated coiled-coil containing kinase 2 Article Snippet: Equal amounts of protein (20 μg) per sample were subjected to SDS-PAGE (30% Acrylamide/Bis Solution, Bio-Rad; 1610158) and transferred to PVDF membranes (Millipore; IPVH00010). .. The PVDF membranes were blocked with 5% nonfat dry milk (Research Products International; M17200 ) in TBST for 1 hour and then incubated overnight at 4°C with primary antibodies against ROCK1 (BD Biosciences; 611136), ROCK2 (BD Biosciences; 610623), phospho-Thr 850 MBS (Millipore; 36-003), total MBS (BioLegend; 925101), UCP1 (Abcam; ab23841), Article Title: HOXA9 orchestrates EMT and metastasis in oral cancer via transcriptional activation of vimentin and β-catenin signaling. Article Snippet: Scrambled and HOXA9-knockdown cells were homogenized in RIPA buffer, and the total proteins were resolved by electrophoresis on a 10% SDS‒polyacrylamide gel and then transferred on a nitrocellulose membrane (Bio-Rad, USA). .. The blotted membranes were blocked with BSA (5%) and incubated with primary antibodies, namely, HOXA9 (1:1000, sc-81291, Santacruz, USA), CTNNB1 (8480T), nonphospho-CTNNB1 (8814S) , SNAI2 (9585T), C-Myc (5605T), TCF-1 (2203T), CASP9 (9502T), CDH1 (3195T), ACTB (4967S), phospho-AKT (9271S), Total-AKT (9272), phospho-ERK1/2 (4370S), Total-ERK1/2 (4695S, Cell Signaling Technology (CST), USA), CCND1 (ab134175, Abcam, UK), CCNE (sc-247, Santacruz, USA), CDH2 (PAB481Hu01), SNAI1 (PAK089Hu01), and VIM (1:3000, PAB040Hu01, Cloud Clone, USA), at 4°C overnight and next day with corresponding secondary antibodies anti-mouse (1:5000, sc-516102, Santa Cruz, USA), or Article Title: LAMC2 Drives Airway Remodeling in COPD via EMT Regulation Through the AKT Pathway Article Snippet: Equal amounts of protein (20–50 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, MA, USA). .. The membranes were blocked with 5% bovine serum albumin for 1 hour at room temperature and then incubated overnight at 4°C with the following primary antibodies: |
